Microscopy

Light microscopy

Stereomicroscopes

Five stereomicroscopes from major microscope manufacturers are available. Generally, these stereomicroscopes are best suited for biological sample observation, and as such are equipped with transmitted light base with different contrasting methods and polarization sets. Auxilliary side light or ring light can be also attached. The objective magnification ranges from 0.8x, through 1.0x and 2.0x, up to 5.0x.

Location: Biology Labs

Widefield fluorescence microscopes

Two high-end Nikon fluorescence microscopes in upright and inverted orientation are available. Wide range of objectives allows for visualization using various contrasting and imaging methods at all magnifications - brightfield, phase contrast, DIC and fluorescence. Filter cubes cover the most commonly used biological fluorophores, such as GFP, YFP, mCherry or DAPI and two two different light sources with specific wavelengths ranges can be used for excitation. Many of the microscope components are interchangable and allow for customizable configurations. For more details, please see the full specification in the list of equipment.

Location: E0.218 (Microscopy BSL2)

 

Confocal microscope

NanoFocus µsurf Custom 200

XY table with 200 mm x 200 mm travel range and a maximum sample height of 100 mm. Confocal microscope with Nipkow disc and a frame rate of 30 Hz and 4MP B/W camera. Objectives:

Magnif FOV W.D. XY res. Z Res.
10x 1600 µm 11.0 mm 1.6 µm 20 nm
20x 800 µm 12.0 mm 0.8 µm 6 nm
50x 320 µm 10.6 mm 0.6 µm 4 nm
50x 320 µm 350 µm 0.4 µm 2 nm
100x 160 µm 3.4 mm 0,4 µm 2 nm

 

Powerful analysis software from digital surf (Mountains Map).

 

 

Digital microscope

Keyence VHX-6000

Material science digital microscope with range of objectives and illumination allowing observation using reflected light, polarized light or DIC.

Location: E0.402 (Dry Sample Preparation)

 

Electron microscopy

Environmental SEM

Quanta FEG 650

Quanta FEG 650

Nano-scale imaging on a wide variety of samples
• Stage 150mm x 150mm
• Samples < 500g
• Hi-vac, low-vac, ESEM-mode
• 100% humidity possible for wet samples
• SE / BSE detectors
• EDS detector
• STEM detector

Location: E0.407 (SEM Lab)

 

FIB/SEM

Helios G4 UC

Dual beam microscope for surface characterization and nanofabrication
• Tomahawk ion-beam column
• Elstar SEM column
• In-lens SE / BSE detectors
• Stage: 150mm x 150mm
• STEM detector
• ICE detector
• EDS detector
• Charge neutralizer
• Fast e-beam blanker
• Auto-TEM lamellar preparation
• Nano-patterning software
• Area-mapping software

 

CryoTEM

JEOL 200 kV TEM JEM-2100F

• Acceleration voltage: 80 - 200 kV
• Electron source: LaB6-cathode
• Best resolution (at 200 kV): Point resolution: 0.27 nm; Line resolution: 0.14 nm
• Camera: Emsis XAROSA (CMOS, 20 Mpix)
• Sample mounting: EM-grids
• Sample preparation: Plunge freezing, Thin slices cut by ultramicrotome

Location: E0.203 (Electron Microscopy Room)

 

Sample preparation

Complementary equipment for EM sample preparation is also available on site:

Sputter coater Leica EM ACE600 for gold/iridium sputtering and carbon thread coating.

Plunger Leica EM GP2 for rapid freezing of samples suspended in liquid.

Ultramicrotome Leica EM7 with cryo-chamber FC7 for ultrathin sectioning.

 

Atomic force microscopy

AFM

JPK NanoWizard 4 BioScience